Coding

Part:BBa_K1831002:Design

Designed by: Morgan Litschko, Malak Elbatarny, Joanna Semrau, Justine Ring   Group: iGEM15_Queens_Canada   (2015-09-11)

T3-10 Self-Assembling Scaffold with K-coil & His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 728
    Illegal XhoI site found at 755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The K-coil was created as an in-frame fusion protein, added to the C-terminus of the T3-10 subunit. By adding a flexible linker of Gly-Ser, between the scaffold and the coil, we incorporated a unique BamHI restriction site into the DNA sequence. This acts as a diagnostic marker for simplified cloning of our BioBrick into the expression vector of your choosing.


Source

This part contains the sequence for the T3-10 subunit (PDB file 4EGG) which was computationally designed by Neil P. King of the Todd O. Yeates & David Baker lab (www.sciencemag.org/cgi/content/full/336/6085/1171/DC1)

References